Coding

Part:BBa_K1336004:Design

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)

Dye-decolourising peroxidase from Pseudomonas putida


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 67
    Illegal NgoMIV site found at 36
    Illegal NgoMIV site found at 673
    Illegal AgeI site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing PCR primers to change the PpDyP gene into the BioBrick format, we also had to design primers for site-directed mutagenesis, as there is 1 illegal restriction site within the native gene: EcoRI at position 67.


Source

From genomic sequence of Pseudomonas putida; PpDyP gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.

References